[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2020-7-13] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EYGA Agar

培養(yǎng)基配方
Composition per liter:
Agar ........................................................................12.0g
K2HPO4 .......................................................................1.1g
Glucose ......................................................................1.0g
Yeast extract.................................................................1.0g
KH2PO4 ......................................................................0.86g
(NH4)2SO4 ....................................................................0.5g
MgSO4 ·7H2O..................................................................0.2g
NaCl..........................................................................0.1g
CaCl2 ......................................................................0.025g
Vitamin B 12 ...............................................................2.0μg
EDTA/trace elements mix......................................................3.0mL
pH 6.8 ± 0.2 at 25°C
EDTA/Trace Elements Mix:
Composition per 600.0mL:
EDTA..........................................................................5.0g
ZnSO4 ·7H2O..................................................................2.2g
MnSO4 ·4H2O.................................................................0.57g
FeSO4 ·7H2O.................................................................0.50g
CoCl2 ·6H2O................................................................0.161g
CuSO4 ·5H2O................................................................0.157g
Na2MoO4 ·2H2O..............................................................0.151g
Preparation of EDTA/Trace Elements Mix:  Add components
to distilled/deionized water and bring volume to 600.0mL. Mix thor-
oughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation and maintenance of Arthrobacter species.