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PHB Delafield Agar
(LMG Medium 123)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://xhztyn.cn

Composition per liter:

Agar ............................................................................................20.0g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Casamino acids.............................................................................0.1g
Ferric ammonium citrate.............................................................0.05g
Yeast extract................................................................................0.05g
Phosphate buffer solution .............................................................1.0L
Solution A/B..........................................................................264.0mL
pH 6.8 ± 0.2 at 25°C
Phosphate Buffer Solution:
Composition per liter:
KH 2 PO 4 .........................................................................................4.5g
Na 2 HPO 4 ·2H 2 O.............................................................................5.8g
Preparation of Phosphate Buffer Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 6.8.
Solution A/B:
Composition per 700.0mL:
Solution A..............................................................................140.0mL
Solution B..............................................................................560.0mL
Solution A:
Composition per 140.0mL:
Poly-β-hydroxybutyrate, powdered............................................1.75g
Preparation of Solution A:  Add poly-β-hydroxybutyrate to distilled/
deionized water and bring volume to 140.0mL. Mix thoroughly. Sonicate
for 30 min. Autoclave for 15 min at 15 psi pressure–121°C. Sonicate again
for 30 min in the sterile bottle. Warm to 50°C.
Solution B:
Composition per 560.0mL:
Agar............................................................................................14.0g
Preparation of Solution B: Add agar to distilled/deionized water
and bring volume to 560.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C.
Preparation of Solution A/B: Aseptically combine 140.0mL of
solution A with 560.0mL of solution B. Warm to 50°C.
Preparation of Medium: Combine components except solution A/
B. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Pour into sterile Petri dishes. Allow agar
to solidify. Bring the Petri dishes to 65°C. Aseptically add 4.0mL of
sterile solution A/B onto the surface of each agar plate.
Use: For the cultivation and maintenance of Pseudomonas lemoignei
Delafield.