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Phenol Nutrient-Supplemented Broth

山東拓普生物工程有限公司 培養(yǎng)基配方 http://xhztyn.cn

Composition per 1000.05mL:

(NH 4 ) 2 SO 4 ....................................................................................2.0g
Na 2 HPO 4 .......................................................................................1.0g
Nutrient broth...........................................................................20.0mL
Phenol (90% solution) .............................................................1.05mL
pH 6.8 ± 0.2 at 25°C
Nutrient Broth:
Composition per liter:
Peptone .........................................................................................5.0g
Beef extract...................................................................................3.0g
Preparation of Nutrient Broth:  Add components to tap water and
bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing.
Preparation of Medium: Add components, except phenol, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add phenol. Mix thoroughly.
Use:  For the cultivation of ATCC strain 1413.