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Phenol Red Agar

山東拓普生物工程有限公司 培養(yǎng)基配方 http://xhztyn.cn

Composition per liter:

Agar ............................................................................................15.0g
Proteose peptone No. 3...............................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................1.0g
Phenol Red................................................................................0.025g
Carbohydrate solution..............................................................20.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Carbohydrate Solution:
Composition per 20.0mL:
Carbohydrate........................................................................5.0–10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 20.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 980.0mL.
Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 20.0mL of
sterile carbohydrate solution. Pour into sterile Petri dishes or distribute
into sterile tubes. Allow tubes to cool in a slanted position.
Use: For the determination of fermentation reactions. Bacteria that can
ferment the added carbohydrate turn the medium yellow.