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Tomato Juice Yeast Extract Milk Medium

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司培養(yǎng)基配方 http://xhztyn.cn

Composition per liter:

Skim milk.................................................................................. 100.0g

Yeast extract.................................................................................. 5.0g

Tomato juice, filtered.............................................................100.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Filter canned tomato juice through paper.

Let stand overnight at 10°C. Add remaining components and bring to

1.0L with distilled/deionized water. Mix thoroughly. Distribute into

tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of a variety of fastidious

bacteria that require complex growth factors, including Lactobacillus,

Streptococcus, and Enterococcus species.

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