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TPYG Medium

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

TPYG Medium

山東拓普生物工程有限公司培養(yǎng)基配方 http://xhztyn.cn

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Peptone.......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

Glucose solution ......................................................................50.0mL

Glucose Solution:

Composition per 100.0mL:

Glucose ....................................................................................... 20.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except glucose solu

tion, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical

ly add 50.0mL of sterile glucose solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation of Clostridium felsineum.

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