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培養(yǎng)基配方

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Transgrow Medium

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Transgrow Medium

山東拓普生物工程有限公司培養(yǎng)基配方 http://xhztyn.cn

Composition per liter:

GC agar base..........................................................................730.0mL

Hemoglobin solution .............................................................250.0mL

Vitox supplement.....................................................................10.0mL

VCN antibiotic solution...........................................................10.0mL

pH 7.3 ± 0.2 at 25°C

GC Agar Base:

Composition per 730.0mL:

Special peptone........................................................................... 15.0g

Agar ............................................................................................ 20.0g

NaCl.............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Cornstarch..................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

pH 7.2 ± 0.2 at 25°C

Preparation of GC Agar Base: Add components of GC medium

base and the hemoglobin to distilled/deionized water and bring volume

to 730.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Hemoglobin Solution:

Composition per 250.0mL:

Hemoglobin .................................................................................. 5.0g

Preparation of Hemoglobin Solution: Add hemoglobin to dis

tilled/deionized water and bring volume to 250.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Vitox Supplement:

Composition per 10.0mL:

Glucose ......................................................................................... 2.0g

L-Cysteine·HCl ......................................................................... 0.518g

L-Glutamine .................................................................................. 0.2g

L-Cystine................................................................................... 0.022g

Adenine sulfate ........................................................................... 0.01g

Nicotinamide adenine dinucleotide ........................................... 5.0mg

Cocarboxylase............................................................................ 2.0mg

Guanine·HCl .............................................................................. 0.6mg

Fe(NO3)3·6H2O.......................................................................... 0.4mg

p-Aminobenzoic acid............................................................... 0.26mg

Vitamin B12 ................................................................................ 0.2mg

Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitox Supplement: Add components to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

VCN Antibiotic Solution:

Composition per 10.0mL:

Colistin methane sulfonate.........................................................7.5mg

Vancomycin ...............................................................................3.0mg

Nystatin.................................................................................. 12,500U

Preparation of VCN Antibiotic Solution: Add components to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: To 730.0mL of cooled, sterile GC agar

base, aseptically add 250.0mL of sterile hemoglobin solution, 10.0mL

of sterile Vitox supplement, and 10.0mL of VCN antibiotic solution.

Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation and transport of fastidious microorganisms,

especially Neisseria species.

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Transgrow Medium